Matrix metalloproteinase 2 is associated with stable and matrix metalloproteinases 8 and 9 with vulnerable carotid atherosclerotic lesions: a study in human …
JPG Sluijter, WPC Pulskens, AH Schoneveld… - Stroke, 2006 - Am Heart Assoc
JPG Sluijter, WPC Pulskens, AH Schoneveld, E Velema, CF Strijder, F Moll, JP de Vries…
Stroke, 2006•Am Heart AssocBackground and Purpose—We studied matrix metalloproteinases (MMP) 2, 8, and 9 and
extracellular matrix metalloproteinase inducer (EMMPRIN) levels in relation to carotid
atherosclerotic plaque characteristics. Methods—Carotid atherosclerotic plaques (n= 150)
were stained and analyzed for the presence of collagen, smooth muscle cell (SMC), and
macrophages. Adjacent segments were used to isolate total protein to assess MMP-2 and
MMP-9 activities and gelatin breakdown, MMP-8 activity, and EMMPRIN levels. Results …
extracellular matrix metalloproteinase inducer (EMMPRIN) levels in relation to carotid
atherosclerotic plaque characteristics. Methods—Carotid atherosclerotic plaques (n= 150)
were stained and analyzed for the presence of collagen, smooth muscle cell (SMC), and
macrophages. Adjacent segments were used to isolate total protein to assess MMP-2 and
MMP-9 activities and gelatin breakdown, MMP-8 activity, and EMMPRIN levels. Results …
Background and Purpose— We studied matrix metalloproteinases (MMP) 2, 8, and 9 and extracellular matrix metalloproteinase inducer (EMMPRIN) levels in relation to carotid atherosclerotic plaque characteristics.
Methods— Carotid atherosclerotic plaques (n=150) were stained and analyzed for the presence of collagen, smooth muscle cell (SMC), and macrophages. Adjacent segments were used to isolate total protein to assess MMP-2 and MMP-9 activities and gelatin breakdown, MMP-8 activity, and EMMPRIN levels.
Results— Macrophage-rich lesions revealed higher MMP-8 and MMP-9 activities, whereas SMC-rich lesions showed higher MMP-2 activity. The levels of less glycosylated EMMPRIN-45kD were higher in SMC-rich lesions and lower in macrophage-rich plaques. EMMPRIN-45kD was associated with MMP-2 levels, whereas EMMPRIN-58kD was related to MMP-9 levels.
Conclusions— MMP-2, MMP-8, and MMP-9 activities differed among carotid plaque phenotypes. Different EMMPRIN glycosylation forms are associated with either MMP-2 or MMP-9 activity, which suggests that EMMPRIN glycosylation may play a role in MMP regulation and plaque destabilization.
Am Heart Assoc