iCLIP-transcriptome-wide mapping of protein-RNA interactions with individual nucleotide resolution

J Konig, K Zarnack, G Rot, T Curk, M Kayikci… - JoVE (Journal of …, 2011 - jove.com
The unique composition and spatial arrangement of RNA-binding proteins (RBPs) on a
transcript guide the diverse aspects of post-transcriptional regulation1. Therefore, an
essential step towards understanding transcript regulation at the molecular level is to gain
positional information on the binding sites of RBPs2. Protein-RNA interactions can be
studied using biochemical methods, but these approaches do not address RNA binding in
its native cellular context. Initial attempts to study protein-RNA complexes in their cellular …