Phenotypic and functional attributes of lentivirus-modified CD19-specific human CD8+ central memory T cells manufactured at clinical scale
X Wang, A Naranjo, CE Brown, C Bautista… - Journal of …, 2012 - journals.lww.com
A key determinant of the therapeutic potency of adoptive T-cell transfer is the extent to which
infused cells can persist and expand in vivo. Ex vivo propagated virus-specific and chimeric
antigen receptor (CAR)-redirected antitumor CD8+ effector T cells derived from CD45RA−
CD62L+ central memory (T CM) precursors engraft long-term and reconstitute functional
memory after adoptive transfer. Here, we describe a clinical scale, closed system,
immunomagnetic selection method to isolate CD8+ T CM from peripheral blood …
infused cells can persist and expand in vivo. Ex vivo propagated virus-specific and chimeric
antigen receptor (CAR)-redirected antitumor CD8+ effector T cells derived from CD45RA−
CD62L+ central memory (T CM) precursors engraft long-term and reconstitute functional
memory after adoptive transfer. Here, we describe a clinical scale, closed system,
immunomagnetic selection method to isolate CD8+ T CM from peripheral blood …