Increased hepatocyte turnover and inhibition of woodchuck hepatitis B virus replication by adefovir in vitro do not lead to reduction of the closed circular DNA
M Dandri, MR Burda, H Will, J Petersen - Hepatology, 2000 - Elsevier
The aim of this study was to evaluate the inhibitory effect of the nucleotide analogue adefovir
on woodchuck hepatitis B virus (WHV) replication and, in particular, to determine whether
the pool of covalently closed circular DNA (cccDNA) could be reduced by adefovir treatment
in primary cultures of woodchuck hepatocytes isolated from a chronic carrier. Strong
reduction of WHV-DNA synthesis (90%) and secretion (up to 98%) was observed with all 3
doses of adefovir used (1, 10, and 100 μmol/L), whereas in the absence of the drug, high …
on woodchuck hepatitis B virus (WHV) replication and, in particular, to determine whether
the pool of covalently closed circular DNA (cccDNA) could be reduced by adefovir treatment
in primary cultures of woodchuck hepatocytes isolated from a chronic carrier. Strong
reduction of WHV-DNA synthesis (90%) and secretion (up to 98%) was observed with all 3
doses of adefovir used (1, 10, and 100 μmol/L), whereas in the absence of the drug, high …