[HTML][HTML] The bromodomain protein Brd4 stimulates G1 gene transcription and promotes progression to S phase
K Mochizuki, A Nishiyama, MK Jang, A Dey… - Journal of biological …, 2008 - ASBMB
Brd4 is a bromodomain protein that binds to acetylated chromatin. It regulates cell growth,
although the underlying mechanism has remained elusive. Brd4 has also been shown to
control transcription of viral genes, whereas its role in transcription of cellular genes has not
been fully elucidated. Here we addressed the role of Brd4 in cell growth and transcription
using a small hairpin (sh) RNA approach. The Brd4 shRNA vector stably knocked down
Brd4 protein expression by∼ 90% in NIH3T3 cells and mouse embryonic fibroblasts. Brd4 …
although the underlying mechanism has remained elusive. Brd4 has also been shown to
control transcription of viral genes, whereas its role in transcription of cellular genes has not
been fully elucidated. Here we addressed the role of Brd4 in cell growth and transcription
using a small hairpin (sh) RNA approach. The Brd4 shRNA vector stably knocked down
Brd4 protein expression by∼ 90% in NIH3T3 cells and mouse embryonic fibroblasts. Brd4 …