[HTML][HTML] Silencing of Cytosolic or Mitochondrial Isoforms of Malic Enzyme Has No Effect on Glucose-stimulated Insulin Secretion from Rodent Islets∗

SM Ronnebaum, MV Jensen, HE Hohmeier… - Journal of biological …, 2008 - ASBMB
We have previously demonstrated a role for pyruvate cycling in glucose-stimulated insulin
secretion (GSIS). Some of the possible pyruvate cycling pathways are completed by
conversion of malate to pyruvate by malic enzyme. Using INS-1-derived 832/13 cells, it has
recently been shown by other laboratories that NADP-dependent cytosolic malic enzyme
(MEc), but not NAD-dependent mitochondrial malic enzyme (MEm), regulates GSIS. In the
current study, we show that small interfering RNA-mediated suppression of either MEm or …