Inducible gene targeting in mice

R Kühn, F Schwenk, M Aguet, K Rajewsky - Science, 1995 - science.org
R Kühn, F Schwenk, M Aguet, K Rajewsky
Science, 1995science.org
A method of gene targeting that allows the inducible inactivation of a target gene in mice is
presented. The method uses an interferon-responsive promoter to control the expression of
Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase β gene
flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly
complete in lymphocytes within a few days, whereas partial deletion was obtained in other
tissues. This method can be used for the inducible inactivation of any other gene in vivo.
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase β gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
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