A minute chromosome in human chronic granulocytic leukemia

P Nowell, D Hungerford - Landmarks in medical genetics …, 2004 - books.google.com
P Nowell, D Hungerford
Landmarks in medical genetics: classic papers with commentaries, 2004books.google.com
104 PART III HUMAN CHROMOSOMES AND THEIR DISORDERS human leukemia
[including two of the seven cases reported here: Nowell and Hungerford, J. Natl. Cancer Inst.
25, 85 (1960)], but no series has appeared in which there was a consistent change typical of
a particular type of leukemia. Cells of the five new cases were obtained from peripheral
blood (and bone marrow in one instance), grown in culture for 24–72 hours, and processed
for cytological examination by a recently developed air-drying technique (Moorhead, et al …
104 PART III HUMAN CHROMOSOMES AND THEIR DISORDERS human leukemia [including two of the seven cases reported here: Nowell and Hungerford, J. Natl. Cancer Inst. 25, 85 (1960)], but no series has appeared in which there was a consistent change typical of a particular type of leukemia. Cells of the five new cases were obtained from peripheral blood (and bone marrow in one instance), grown in culture for 24–72 hours, and processed for cytological examination by a recently developed air-drying technique (Moorhead, et al., Exptl. Cell Research, in press). The patients varied from asymptoyears’ duration in terminal myeloblastic crisis. All seven individuals showed a similar minute chromosome, and none showed any other frequent or regular chromosome change. In most of the cases, cells with normal chromosomes were also observed. Thus, the minute is not a part of the normal chromosome constitution of such individuals. The findings suggest a causal relationship between the chromosome abnormality observed and chronic granulocytic leukemia.
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