[HTML][HTML] Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics

T Kitamura, Y Koshino, F Shibata, T Oki… - Experimental …, 2003 - Elsevier
Most of the human genome has now been sequenced and about 30,000 potential open
reading frames have been identified, indicating that we use these 30,000 genes to
functionally organize our biologic activities. However, functions of many genes are still
unknown despite intensive efforts using bioinformatics as well as transgenic and knockout
mice. Retrovirus-mediated gene transfer is a powerful tool that can be used to understand
gene functions. We have developed a variety of retrovirus vectors and efficient packaging …