New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins
J Ko, S Ou, PH Patterson - Brain research bulletin, 2001 - Elsevier
We produced eight anti-huntingtin (Htt) monoclonal antibodies (mAbs), several of which
have novel binding patterns. Peptide array epitope mapping shows that mAbs MW1–6
specifically bind the polyQ domain of Htt exon 1. On Western blots of extracts from mutant Htt
knock-in mouse brain and Huntington's disease lymphoblastoma cell lines, MW1-5 all
strongly prefer to bind to the expanded polyQ repeat form of Htt, displaying no detectable
binding to normal Htt. These results suggest that the polyQ domain can assume different …
have novel binding patterns. Peptide array epitope mapping shows that mAbs MW1–6
specifically bind the polyQ domain of Htt exon 1. On Western blots of extracts from mutant Htt
knock-in mouse brain and Huntington's disease lymphoblastoma cell lines, MW1-5 all
strongly prefer to bind to the expanded polyQ repeat form of Htt, displaying no detectable
binding to normal Htt. These results suggest that the polyQ domain can assume different …