Image-based high-throughput quantification of cellular fat accumulation

M Dragunow, R Cameron, P Narayan, S O'Carroll - SLAS Discovery, 2007 - Elsevier
A number of biochemical methods are available for measuring fat accumulation in cell
culture. The authors report a simple image-based method for measuring fat accumulation in
adipocytes using a combination of high-throughput brightfield microscopy and image
analysis, which was validated biochemically using Oil-Red-O. The quickest and most
accurate method of analysis was one based on thresholding brightfield images and
determining the area of fat droplets per image. Thus, the authors have developed a simple …