[HTML][HTML] Mutation of protease domain residues Lys37-39 in human protein C inhibits activation by the thrombomodulin-thrombin complex without affecting activation by …
B Gerlitz, BW Grinnell - Journal of Biological Chemistry, 1996 - ASBMB
Activated protein C (aPC) is an important feedback regulator of the clotting cascade. In vivo,
the conversion of protein C (PC) from its zymogen to activated form is mediated primarily by
thrombin bound to thrombomodulin (TM), an endothelial cell surface protein. Molecular
modeling suggests residues Lys 37-Lys 38-Lys 39 of protein C's serine protease domain
reside in a surface-exposed loop (variable region 1) whose high concentration of positive
charge might be involved in protein-protein interactions. In this study, we have examined the …
the conversion of protein C (PC) from its zymogen to activated form is mediated primarily by
thrombin bound to thrombomodulin (TM), an endothelial cell surface protein. Molecular
modeling suggests residues Lys 37-Lys 38-Lys 39 of protein C's serine protease domain
reside in a surface-exposed loop (variable region 1) whose high concentration of positive
charge might be involved in protein-protein interactions. In this study, we have examined the …