The number of Toll-like receptor 9-agonist motifs in the adenovirus genome correlates with induction of dendritic cell maturation by adenovirus immune complexes

M Perreau, HC Welles, C Pellaton, B Gjoksi… - Journal of …, 2012 - Am Soc Microbiol
M Perreau, HC Welles, C Pellaton, B Gjoksi, L Potin, R Martin, A Harari, A Bett, D Casimiro…
Journal of virology, 2012Am Soc Microbiol
ABSTRACT Adenovirus serotype 5 (Ad5) vectors and specific neutralizing antibodies (NAbs)
generate immune complexes (ICs) which are potent inducers of dendritic cell (DC)
maturation. Here we show that ICs generated with rare Ad vector serotypes, such as Ad26
and Ad35, which are lead candidates in HIV vaccine development, are poor inducers of DC
maturation and that their potency in inducing DC maturation strongly correlated with the
number of Toll-like receptor 9 (TLR9)-agonist motifs present in the Ad vector's genome. In …
Abstract
Adenovirus serotype 5 (Ad5) vectors and specific neutralizing antibodies (NAbs) generate immune complexes (ICs) which are potent inducers of dendritic cell (DC) maturation. Here we show that ICs generated with rare Ad vector serotypes, such as Ad26 and Ad35, which are lead candidates in HIV vaccine development, are poor inducers of DC maturation and that their potency in inducing DC maturation strongly correlated with the number of Toll-like receptor 9 (TLR9)-agonist motifs present in the Ad vector's genome. In addition, we showed that antihexon but not antifiber antibodies are responsible for the induction of Ad IC-mediated DC maturation.
American Society for Microbiology