[HTML][HTML] Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor
M Hirsila, P Koivunen, V Gunzler, KI Kivirikko… - Journal of Biological …, 2003 - ASBMB
The hypoxia-inducible factors (HIFs) play a central role in oxygen homeostasis.
Hydroxylation of one or two critical prolines by specific hydroxylases (P4Hs) targets their HIF-
α subunits for proteasomal degradation. By studying the three human HIF-P4Hs, we found
that the longest and shortest isoenzymes have major transcripts encoding inactive
polypeptides, which suggest novel regulation by alternative splicing. Recombinant HIF-
P4Hs expressed in insect cells required peptides of more than 8 residues, distinct …
Hydroxylation of one or two critical prolines by specific hydroxylases (P4Hs) targets their HIF-
α subunits for proteasomal degradation. By studying the three human HIF-P4Hs, we found
that the longest and shortest isoenzymes have major transcripts encoding inactive
polypeptides, which suggest novel regulation by alternative splicing. Recombinant HIF-
P4Hs expressed in insect cells required peptides of more than 8 residues, distinct …