Dissection, plating, and maintenance of dorsal root ganglion neurons for monoculture and for coculture with dorsal horn neurons
C Albuquerque, DJ Joseph… - Cold Spring Harbor …, 2009 - cshprotocols.cshlp.org
C Albuquerque, DJ Joseph, P Choudhury, AB MacDermott
Cold Spring Harbor Protocols, 2009•cshprotocols.cshlp.orgDISCUSSION Primary neuronal cultures have been used extensively to characterize the
functional properties of synapses and to study the synaptic effects of pharmacological
agents, proteins, and genetic modification. Coculture preparations comprised of dissociated
DRG and DH neurons have been used as a model system for synaptic transmission at
primary afferent synapses. Additionally, this model permits simultaneous measurement of
post-synaptic response from multiple cell populations in response to DRG activation.
functional properties of synapses and to study the synaptic effects of pharmacological
agents, proteins, and genetic modification. Coculture preparations comprised of dissociated
DRG and DH neurons have been used as a model system for synaptic transmission at
primary afferent synapses. Additionally, this model permits simultaneous measurement of
post-synaptic response from multiple cell populations in response to DRG activation.
DISCUSSION
Primary neuronal cultures have been used extensively to characterize the functional properties of synapses and to study the synaptic effects of pharmacological agents, proteins, and genetic modification. Coculture preparations comprised of dissociated DRG and DH neurons have been used as a model system for synaptic transmission at primary afferent synapses. Additionally, this model permits simultaneous measurement of post-synaptic response from multiple cell populations in response to DRG activation.
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