Commitment of bone marrow cells to hepatic stellate cells in mouse

S Baba, H Fujii, T Hirose, K Yasuchika, H Azuma… - Journal of …, 2004 - Elsevier
Background/Aims: Recently, several cells found within the liver have been reported to derive
from bone marrow (BM). This study sought to examine the commitment of BM cells to hepatic
stellate cell (HSC) lineage in mouse liver. Methods: We transplanted BM cells from green
fluorescent protein (GFP) transgenic mice into age-matched C57BL/J mice. Hepatic
nonparenchymal cells were isolated from the livers of BM-transplanted mice using density
gradient centrifugation with Nycodenz. The expression of lineage markers by the isolated …