Functional analysis of PKD1 transgenic lines reveals a direct role for polycystin-1 in mediating cell-cell adhesion
AJ Streets, LJ Newby, MJ O'Hare… - Journal of the …, 2003 - journals.lww.com
The PKD1 protein, polycystin-1, is a large transmembrane protein of uncertain function and
topology. To study the putative functions of polycystin-1, conditionally immortalized kidney
cells transgenic for PKD1 were generated and an interaction between transgenic polycystin-
1 and endogenous polycystin-2 has been recently demonstrated in these cells. This study
provides the first functional evidence that transgenic polycystin-1 directly mediates cell-cell
adhesion. In non-permeabilized cells, polycystin-1 localized to the lateral cell borders with N …
topology. To study the putative functions of polycystin-1, conditionally immortalized kidney
cells transgenic for PKD1 were generated and an interaction between transgenic polycystin-
1 and endogenous polycystin-2 has been recently demonstrated in these cells. This study
provides the first functional evidence that transgenic polycystin-1 directly mediates cell-cell
adhesion. In non-permeabilized cells, polycystin-1 localized to the lateral cell borders with N …