[HTML][HTML] Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm

A Kihara, M Ikeda, Y Kariya, EY Lee, YM Lee… - Journal of Biological …, 2003 - ASBMB
Sphingosine 1-phosphate (S1P) is a bioactive lipid molecule that acts both extracellularly
and intracellularly. TheSPL gene encodes a mammalian S1P lyase that degrades S1P.
Here, we have disrupted the SPL gene in mouse F9 embryonal carcinoma cells by gene
targeting. This is the first report of gene disruption of mammalian S1P lyase. The SPL-null
cells exhibited no S1P lyase activity, and intracellular S1P was increased∼ 2-fold,
compared with wild-type cells. Treatment of F9 embryonal carcinoma cells with retinoic acid …