Hepatocyte-specific deletion of heme oxygenase-1 disrupts redox homeostasis in basal and oxidative environments

T Mamiya, F Katsuoka, A Hirayama… - The Tohoku journal of …, 2008 - jstage.jst.go.jp
T Mamiya, F Katsuoka, A Hirayama, O Nakajima, A Kobayashi, JM Maher, H Matsui, I Hyodo…
The Tohoku journal of experimental medicine, 2008jstage.jst.go.jp
The full details of conditional targeting strategy of HO-1 will be reported separately (Hosoya
et al. in preparation). Briefly, a floxed HO-1 targeting vector was constructed with a loxP site
inserted into the second intron and a floxed Neo cassette inserted downstream of the
terminal exon. Homologous recombination in 129Svderived embryonic stem cells was
conducted using standardized procedures (Hosoya et al. 2001). Chimeric mice were
crossed with C57BL/6 wild-type mice to generate HO-1flox/+ mice, and the resulting mice …
The full details of conditional targeting strategy of HO-1 will be reported separately (Hosoya et al. in preparation). Briefly, a floxed HO-1 targeting vector was constructed with a loxP site inserted into the second intron and a floxed Neo cassette inserted downstream of the terminal exon. Homologous recombination in 129Svderived embryonic stem cells was conducted using standardized procedures (Hosoya et al. 2001). Chimeric mice were crossed with C57BL/6 wild-type mice to generate HO-1flox/+ mice, and the resulting mice were further crossed with Sycp1-Cre mice (Vidal et al. 1998; generously supplied from the Jackson Laboratory (JAX)) to generate HO-1+/–mice. Albumin-Cre transgenic (Alb-Cre) mice (Postic et al. 1999), which specifically express Cre recombinase in hepatocytes, were also supplied from JAX and crossed with HO-1+/–mice to obtain Alb-Cre: HO-1+/–mice, which were then crossed with HO-1flox/+ mice to generate hepatocyte-specific HO-1-deficient
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