Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation

C Cutler, P Multani, D Robbins, HT Kim… - Blood, The Journal …, 2013 - ashpublications.org
C Cutler, P Multani, D Robbins, HT Kim, T Le, J Hoggatt, LM Pelus, C Desponts, YB Chen
Blood, The Journal of the American Society of Hematology, 2013ashpublications.org
Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) for use
in allogeneic transplantation. Key advantages of UCB are rapid availability and less
stringent requirements for HLA matching. However, UCB contains an inherently limited HSC
count, which is associated with delayed time to engraftment, high graft failure rates, and
early mortality. 16, 16-Dimethyl prostaglandin E2 (dmPGE2) was previously identified to be
a critical regulator of HSC homeostasis, and we hypothesized that brief ex vivo modulation …
Abstract
Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) for use in allogeneic transplantation. Key advantages of UCB are rapid availability and less stringent requirements for HLA matching. However, UCB contains an inherently limited HSC count, which is associated with delayed time to engraftment, high graft failure rates, and early mortality. 16,16-Dimethyl prostaglandin E2 (dmPGE2) was previously identified to be a critical regulator of HSC homeostasis, and we hypothesized that brief ex vivo modulation with dmPGE2 could improve patient outcomes by increasing the “effective dose” of HSCs. Molecular profiling approaches were used to determine the optimal ex vivo modulation conditions (temperature, time, concentration, and media) for use in the clinical setting. A phase 1 trial was performed to evaluate the safety and therapeutic potential of ex vivo modulation of a single UCB unit using dmPGE2 before reduced-intensity, double UCB transplantation. Results from this study demonstrated clear safety with durable, multilineage engraftment of dmPGE2-treated UCB units. We observed encouraging trends in efficacy, with accelerated neutrophil recovery (17.5 vs 21 days, P = .045), coupled with preferential, long-term engraftment of the dmPGE2-treated UCB unit in 10 of 12 treated participants. This study was registered at www.clinicaltrials.gov as #NCT00890500.
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