Figure 1 Generation of TALEn-mediated Pibf1 mutant mice.(a) DnA-binding sequences (in red or blue) and the spacer region for Pibf1-TALEn.(b) T7 endonuclease I (T7E1) assays were conducted using genomic DnA from pronuclear-stage embryos intra-cytoplasmically injected with Pibf1-TALEN mRnA (50 ng/µl). The arrow indicates the size (~ 190 bp) of T7E1-digested DnA fragments.* Cannibalized mice.(c) DnA sequences of thePibf1 locus from live F0 mice identified by T7E1 assays in b.‘-’denotes deleted nucleotides. Underlined sequences in lower case represent nucleotide substitutions.