Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

SW Cho, S Kim, JM Kim, JS Kim - Nature biotechnology, 2013 - nature.com
SW Cho, S Kim, JM Kim, JS Kim
Nature biotechnology, 2013nature.com
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-
guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in
human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs
efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
Abstract
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
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