Generation of functional human vascular network

T Takebe, N Koike, K Sekine, M Enomura… - Transplantation …, 2012 - Elsevier
BACKGROUND: One of the major obstacles in regenerating thick, complex tissues such as
the liver is their need for vascularization, which is essential to maintain cell viability during
tissue growth and to induce structural organization. Herein, we have described a method to
engineer a functional human vascular network. METHODS: Enhanced green fluorescence
protein-labeled human umbilical vein endothelial cells (GFP-HUVECs) were cocultivated
with kusabira orange-labeled human mesenchymal stem cells (KO-hMSCs) inside a …