[HTML][HTML] Deletion of the carboxyl terminus of Tie2 enhances kinase activity, signaling, and function: evidence for an autoinhibitory mechanism

XL Niu, KG Peters, CD Kontos - Journal of Biological Chemistry, 2002 - ASBMB
Tie2 is an endothelial receptor tyrosine kinase that is required for both embryonic vascular
development and tumor angiogenesis. There is considerable interest in understanding the
mechanisms of Tie2 activation for therapeutic purposes. The recent solution of the Tie2
crystal structure suggests that Tie2 activity is autoinhibited by its carboxyl terminus. Here we
investigated the role of the C tail in Tie2 activation, signaling, and function both in vitro and
in vivo by deleting the C terminus of Tie2 (ΔCT). Compared to wild type Tie2, in …