[HTML][HTML] Fatty acids suppress autophagic turnover in β-cells
G Las, SB Serada, JD Wikstrom, G Twig… - Journal of Biological …, 2011 - ASBMB
Recent studies have shown that autophagy is essential for proper β-cell function and
survival. However, it is yet unclear under what pathogenic conditions autophagy is inhibited
in β-cells. Here, we report that long term exposure to fatty acids and glucose block
autophagic flux in β-cells, contributing to their toxic effect. INS1 cells expressing GFP-LC3
(an autophagosome marker) were treated with 0.4 mm palmitate, 0.4 mm oleate, and various
concentrations of glucose for 22 h. Kinetics of the effect of fatty acids on autophagy showed …
survival. However, it is yet unclear under what pathogenic conditions autophagy is inhibited
in β-cells. Here, we report that long term exposure to fatty acids and glucose block
autophagic flux in β-cells, contributing to their toxic effect. INS1 cells expressing GFP-LC3
(an autophagosome marker) were treated with 0.4 mm palmitate, 0.4 mm oleate, and various
concentrations of glucose for 22 h. Kinetics of the effect of fatty acids on autophagy showed …