Isolation and characterization of human salivary gland cells for stem cell transplantation to reduce radiation-induced hyposalivation
J Feng, M van der Zwaag, MA Stokman… - Radiotherapy and …, 2009 - Elsevier
BACKGROUND: Recently, we showed that transplantation of 100–300 c-Kit+ stem cells
isolated from cultured salispheres ameliorates radiation-damage in murine salivary glands.
The aim of this study is to optimize and translate these findings from mice to man.
METHODS: Mouse and human non-malignant parotid and submandibular salivary gland
tissue was collected and enzymatically digested. The remaining cell suspension was
cultured according to our salisphere culture method optimized for murine salispheres …
isolated from cultured salispheres ameliorates radiation-damage in murine salivary glands.
The aim of this study is to optimize and translate these findings from mice to man.
METHODS: Mouse and human non-malignant parotid and submandibular salivary gland
tissue was collected and enzymatically digested. The remaining cell suspension was
cultured according to our salisphere culture method optimized for murine salispheres …