Mechanisms of oxygen glucose deprivation-induced glutamate release from cerebrocortical slice cultures
S Fujimoto, H Katsuki, T Kume, S Kaneko… - Neuroscience …, 2004 - Elsevier
Glutamate has been recognized to mediate ischemia-induced neuronal injury in the brain,
but the source of extracellular glutamate during ischemic insults remains controversial. We
investigated the mechanisms of glutamate release in organotypic cerebrocortical slice
cultures prepared from rat neonates, using oxygen glucose deprivation (OGD) as an in vitro
ischemia model. Slice cultures were submerged in glucose-free deoxygenated buffer for 20–
60min and glutamate released into the extracellular buffer was quantified. Cell injury was …
but the source of extracellular glutamate during ischemic insults remains controversial. We
investigated the mechanisms of glutamate release in organotypic cerebrocortical slice
cultures prepared from rat neonates, using oxygen glucose deprivation (OGD) as an in vitro
ischemia model. Slice cultures were submerged in glucose-free deoxygenated buffer for 20–
60min and glutamate released into the extracellular buffer was quantified. Cell injury was …
