Dicer is essential for mouse development
E Bernstein, SY Kim, MA Carmell, EP Murchison… - Nature …, 2003 - nature.com
Nature genetics, 2003•nature.com
To address the biological function of RNA interference (RNAi)-related pathways in
mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in
development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to
generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by
implication, the RNAi machinery, in maintaining the stem cell population during early mouse
development.
mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in
development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to
generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by
implication, the RNAi machinery, in maintaining the stem cell population during early mouse
development.
Abstract
To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.
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