[HTML][HTML] Putative N-acylphosphatidylethanolamine synthase from Arabidopsis thaliana is a lysoglycerophospholipid acyltransferase
E Bulat, TA Garrett - Journal of Biological Chemistry, 2011 - ASBMB
AT1G78690, a gene found in Arabidopsis thaliana, has been reported to encode a N-
acyltransferase that transfers an acyl chain from acyl-CoA to the headgroup of
phosphatidylethanolamine (PE) to form N-acylphosphatidylethanolamine (N-acyl-PE). Our
investigation suggests that At1g78690p is not a PE-dependent N-acyltransferase but is
instead a lysoglycerophospholipid O-acyltransferase. We overexpressed AT1G78690 in
Escherichia coli, extracted the cellular lipids, and identified the accumulating …
acyltransferase that transfers an acyl chain from acyl-CoA to the headgroup of
phosphatidylethanolamine (PE) to form N-acylphosphatidylethanolamine (N-acyl-PE). Our
investigation suggests that At1g78690p is not a PE-dependent N-acyltransferase but is
instead a lysoglycerophospholipid O-acyltransferase. We overexpressed AT1G78690 in
Escherichia coli, extracted the cellular lipids, and identified the accumulating …