[HTML][HTML] GLUT4 overexpression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4 vesicles and the subcellular localization of GLUT4 and …
E Carvalho, SE Schellhorn, JM Zabolotny… - Journal of Biological …, 2004 - ASBMB
The majority of GLUT4 is sequestered in unique intracellular vesicles in the absence of
insulin. Upon insulin stimulation GLUT4 vesicles translocate to, and fuse with, the plasma
membrane. To determine the effect of GLUT4 content on the distribution and subcellular
trafficking of GLUT4 and other vesicle proteins, adipocytes of adipose-specific, GLUT4-
deficient (aP2-GLUT4-/-) mice and adipose-specific, GLUT4-overexpressing (aP2-GLUT4-
Tg) mice were studied. GLUT4 amount was reduced by 80-95% in aP2-GLUT4-/-adipocytes …
insulin. Upon insulin stimulation GLUT4 vesicles translocate to, and fuse with, the plasma
membrane. To determine the effect of GLUT4 content on the distribution and subcellular
trafficking of GLUT4 and other vesicle proteins, adipocytes of adipose-specific, GLUT4-
deficient (aP2-GLUT4-/-) mice and adipose-specific, GLUT4-overexpressing (aP2-GLUT4-
Tg) mice were studied. GLUT4 amount was reduced by 80-95% in aP2-GLUT4-/-adipocytes …