[HTML][HTML] Phosphatidic acid and N-acylphosphatidylethanolamine form membrane domains in Escherichia coli mutant lacking cardiolipin and phosphatidylglycerol
E Mileykovskaya, AC Ryan, X Mo, CC Lin… - Journal of Biological …, 2009 - ASBMB
The pgsA null Escherichia coli strain, UE54, lacks the major anionic phospholipids
phosphatidylglycerol and cardiolipin. Despite these alterations the strain exhibits relatively
normal cell division. Analysis of the UE54 phospholipids using negativeion electrospray
ionization mass spectrometry resulted in identification of a new anionic phospholipid, N-
acylphosphatidylethanolamine. Staining with the fluorescent dye 10-N-nonyl acridine
orange revealed anionic phospholipid membrane domains at the septal and polar regions …
phosphatidylglycerol and cardiolipin. Despite these alterations the strain exhibits relatively
normal cell division. Analysis of the UE54 phospholipids using negativeion electrospray
ionization mass spectrometry resulted in identification of a new anionic phospholipid, N-
acylphosphatidylethanolamine. Staining with the fluorescent dye 10-N-nonyl acridine
orange revealed anionic phospholipid membrane domains at the septal and polar regions …