Herein we describe a protocol to deliver various reagents to the mouse mammary gland via
intraductal injections. Localized drug delivery and knock-down of genes within the mammary
epithelium has been difficult to achieve due to the lack of appropriate targeting molecules
that are independent of developmental stages such as pregnancy and lactation. Herein, we
describe a technique for localized delivery of reagents to the mammary gland at any stage in
adulthood via intraductal injection into the nipples of mice. The injections can be performed …