Androgen-independent cancer progression and bone metastasis in the LNCaP model of human prostate cancer

GN Thalmann, PE Anezinis, SM Chang, HE Zhau… - Cancer research, 1994 - AACR
GN Thalmann, PE Anezinis, SM Chang, HE Zhau, EE Kim, VL Hopwood, S Pathak…
Cancer research, 1994AACR
Our laboratory has previously reported on the derivation of LNCaP cell sublines from LNCaP
tumors maintained in castrated and intact athymic male mice. These LNCaP sublines differ
from the parental line in tumorigenicity and androgen dependence. This paper demonstrates
that one of these sublines acquired metastatic potential. When inoculated either sc or
orthotopically, the C4-2 subline metastasized to the lymph node and bone with an incidence
of 11–50%. Interestingly, the incidence of osseous metastasis was higher in castrated than …
Abstract
Our laboratory has previously reported on the derivation of LNCaP cell sublines from LNCaP tumors maintained in castrated and intact athymic male mice. These LNCaP sublines differ from the parental line in tumorigenicity and androgen dependence. This paper demonstrates that one of these sublines acquired metastatic potential. When inoculated either s.c. or orthotopically, the C4-2 subline metastasized to the lymph node and bone with an incidence of 11–50%. Interestingly, the incidence of osseous metastasis was higher in castrated than in intact male hosts. We evaluated the chromosomal, immunohistochemical, and biochemical characteristics of the LNCaP sublines derived from C4-2 tumors that metastasized to the lymph node and bone. Cytogenetic analysis showed that all sublines were human and shared common marker chromosomes with the parental LNCaP cells. This experimental human prostate cancer model may permit, for the first time, the study of the molecular mechanisms underlying human prostate cancer metastasis.
AACR