[HTML][HTML] A single site (Ser16) phosphorylation in phospholamban is sufficient in mediating its maximal cardiac responses to β-agonists

G Chu, JW Lester, KB Young, W Luo, J Zhai… - Journal of Biological …, 2000 - ASBMB
Phospholamban (PLB) can be phosphorylated at Ser 16 by cyclic AMP-dependent protein
kinase and at Thr 17 by Ca 2+-calmodulin-dependent protein kinase during β-agonist
stimulation. A previous study indicated that mutation of S16A in PLB resulted in lack of Thr
17 phosphorylation and attenuation of the β-agonist stimulatory effects in perfused mouse
hearts. To further delineate the functional interplay between dual-site PLB phosphorylation,
we generated transgenic mice expressing the T17A mutant PLB in the cardiac compartment …