Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay

L Wu, JG Belasco - Methods in enzymology, 2008 - Elsevier
MicroRNAs (miRNAs) utilize multiple posttranscriptional mechanisms to downregulate gene
expression in metazoan organisms. These include translation repression and accelerated
mRNA decay, the latter being triggered either by deadenylation or, less frequently, by
endonucleolytic cleavage, as governed by the degree of complementarity of the targeted
message. This chapter describes methods for examining the effect of miRNAs on the
translation and turnover of complementary mRNAs in cultured mammalian cells. Among …