[HTML][HTML] Human sterol 27-hydroxylase (CYP27) overexpressor transgenic mouse model: evidence against 27-hydroxycholesterol as a critical regulator of cholesterol …
K Meir, D Kitsberg, I Alkalay, F Szafer, H Rosen… - Journal of Biological …, 2002 - ASBMB
CYP27-overexpressed transgenic mice were generated with the use of a human full-length
CYP27 coding region cloned into a ubiquitous expression vector. Positive transgenic mice
were identified by tail DNA genotyping and high fecal 27-hydroxycholesterol content. The
levels of 27-hydroxycholesterol were found to be 3–5 times higher in the circulation and the
tissues of the overexpressed mice when compared with littermate controls. There were no
gross morphological differences between the overexpressed mice and their controls. Total …
CYP27 coding region cloned into a ubiquitous expression vector. Positive transgenic mice
were identified by tail DNA genotyping and high fecal 27-hydroxycholesterol content. The
levels of 27-hydroxycholesterol were found to be 3–5 times higher in the circulation and the
tissues of the overexpressed mice when compared with littermate controls. There were no
gross morphological differences between the overexpressed mice and their controls. Total …