Secreted interleukin-1α induces a metastatic phenotype in pancreatic cancer by sustaining a constitutive activation of nuclear factor-κB

D Melisi, J Niu, Z Chang, Q Xia, B Peng… - Molecular Cancer …, 2009 - AACR
D Melisi, J Niu, Z Chang, Q Xia, B Peng, S Ishiyama, DB Evans, PJ Chiao
Molecular Cancer Research, 2009AACR
Transcription factor nuclear factor-κB (NF-κB) is constitutively activated in most pancreatic
cancer tissues and cell lines but not in normal pancreas nor in immortalized/nontumorigenic
human pancreatic ductal epithelial cells. Inhibition of constitutive NF-κB activation in
pancreatic cancer cell lines suppresses tumorigenesis and tumor metastasis. Recently, we
identified autocrine secretion of proinflammatory cytokine interleukin (IL)-1α as the
mechanism of constitutive NF-κB activation in metastatic pancreatic cancer cell lines …
Abstract
Transcription factor nuclear factor-κB (NF-κB) is constitutively activated in most pancreatic cancer tissues and cell lines but not in normal pancreas nor in immortalized/nontumorigenic human pancreatic ductal epithelial cells. Inhibition of constitutive NF-κB activation in pancreatic cancer cell lines suppresses tumorigenesis and tumor metastasis. Recently, we identified autocrine secretion of proinflammatory cytokine interleukin (IL)-1α as the mechanism of constitutive NF-κB activation in metastatic pancreatic cancer cell lines. However, the role of IL-1α in determining the metastatic potential of pancreatic tumor remains to be further investigated. In the current study, we stably expressed IL-1α in the nonmetastatic, IL-1α–negative MiaPaCa-2 cell lines. Our results showed that the secretion of IL-1α in MiaPaCa-2 cells constitutively activated NF-κB and increased the expression of NF-κB downstream genes involved in the different steps of the metastatic cascade, such as urokinase-type plasminogen activator, vascular endothelial growth factor, and IL-8. MiaPaCa-2/IL-1α cells showed an enhanced cell invasion in vitro compared with parental MiaPaCa-2 cells and induced liver metastasis in an orthotopic mouse model. The metastatic phenotype induced by IL-1α was inhibited by the expression of phosphorylation-defective IκB (IκB S32, 36A), which blocked NF-κB activation. Consistently, silencing the expression of IL-1α by short hairpin RNA in the highly metastatic L3.6pl pancreatic cancer cells completely suppressed their metastatic spread. In summary, these findings showed that IL-1α plays key roles in pancreatic cancer metastatic behavior through the constitutive activation of NF-κB. Our findings further support the possible link between inflammation and cancer and suggest that IL-1α may be a potential therapeutic target for treating pancreatic adenocarcinoma. (Mol Cancer Res 2009;7(5):624–33)
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