[HTML][HTML] Extracellular regulated kinases (ERK) 1 and ERK2 are authentic substrates for the dual-specificity protein-tyrosine phosphatase VHR: a novel role in down …

JL Todd, KG Tanner, JM Denu - Journal of Biological Chemistry, 1999 - ASBMB
The mammalian dual-specificity protein-tyrosine phosphatase VHR (forVH1-related) has
been identified as a novel regulator of extracellular regulated kinases (ERKs). To identify
potential cellular substrates of VHR, covalently immobilized mutant VHR protein was
employed as an affinity trap. A tyrosine-phosphorylated protein (s) of∼ 42 kDa was
specifically adsorbed by the affinity column and identified as ERK1 and ERK2. Subsequent
kinetic analyses and transfection studies demonstrated that VHR specifically …