In this study, 10 patients aged 4-14 years with cow's milk allergy, referred to Gifu University Hospital in Gifu, Japan, were subjected to a slow dose-up protocol for oral tolerance induction. The initial dose of the slow dose-up method was 1 daily drop of cow's milk (approximately 0.025 ml) in 20 ml of water. The dose was increased every 2 weeks. The challenge was performed at the university hospital under medical supervision. When allergic symptoms appeared, the daily dose was not increased and the previous dose was repeated. The children were then carefully assessed. Overall, 8 patients completed the protocol and were able to tolerate 100 ml of cow's milk. The two patients who did not complete the protocol experienced allergic symptoms, one with urticaria following a 5-ml dose and the other with perioral exanthema following a 20-ml dose. In these two patients, the daily dose of cow's milk was not increased and the last dose was repeated after 2 weeks. The same symptoms reappeared with each repeated dose. Therefore, the patients could not continue the protocol. Three patients experienced mild side effects, including perioral exanthema, which did not require medication or dose reduction. Thus, the described protocol for specific oral tolerance induction can be effectively used for the treatment of children with cow's milk allergy.