Bone embedding in pure methyl methacrylate at low temperature preserves enzyme activities

D Chappard, S Palle, C Alexandre, L Vico, G Riffat - Acta histochemica, 1987 - Elsevier
Summary Pure Methyl Methacrylate (MMA), a widely used embedding medium for
undecalcified bone studies, was polymerized at low temperature (4° C). MMA was prepared
by a new purification procedure yielding a absolutely anhydrous and catalysed resin. The
redox system benzoyle peroxide NN-Dimethylanilin was used as the catalyser-initiator
system providing free radicals for the MMA chemical polymerization. Since the reaction is
inhibited at-20° C, complete infiltration of blocks is achieved within 3 d. Polymerization took …