[HTML][HTML] The small GTPase Rap1 is required for Mn2+-and antibody-induced LFA-1-and VLA-4-mediated cell adhesion
KMT de Bruyn, S Rangarajan, KA Reedquist… - Journal of Biological …, 2002 - ASBMB
In T-lymphocytes the Ras-like small GTPase Rap1 plays an essential role in stimulus-
induced inside-out activation of integrin LFA-1 (α L β 2) and VLA-4 (α 4 β 1). Here we show
that Rap1 is also involved in the direct activation of these integrins by divalent cations or
activating antibodies. Inhibition of Rap1 either by Rap GTPase-activating protein (RapGAP)
or the Rap1 binding domain of RalGDS abolished both Mn 2+-and KIM185 (anti-LFA-1)-
induced LFA-1-mediated cell adhesion to intercellular adhesion molecule 1. Mn 2+-and …
induced inside-out activation of integrin LFA-1 (α L β 2) and VLA-4 (α 4 β 1). Here we show
that Rap1 is also involved in the direct activation of these integrins by divalent cations or
activating antibodies. Inhibition of Rap1 either by Rap GTPase-activating protein (RapGAP)
or the Rap1 binding domain of RalGDS abolished both Mn 2+-and KIM185 (anti-LFA-1)-
induced LFA-1-mediated cell adhesion to intercellular adhesion molecule 1. Mn 2+-and …