Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform
Endosialin/TEM1 is predominantly expressed on neovasculature, thus ideally suited for
diagnostic, targeted imaging and therapy of cancer. To isolate TEM1-specific affinity
reagents, we thought to screen a recombinant antibody (scFv) library derived from the
repertoire of a patient with thrombotic thrombocytopenic purpura (TTP), as autoimmune
disorders may produce self-reactive specificities. The yeast-display scFv library was
constructed by homologous recombination of the TTP patient repertoire originally expressed …
diagnostic, targeted imaging and therapy of cancer. To isolate TEM1-specific affinity
reagents, we thought to screen a recombinant antibody (scFv) library derived from the
repertoire of a patient with thrombotic thrombocytopenic purpura (TTP), as autoimmune
disorders may produce self-reactive specificities. The yeast-display scFv library was
constructed by homologous recombination of the TTP patient repertoire originally expressed …