Although bulk protein turnover has been measured with the use of stable isotope labeled
tracers for over half a century, it is only recently that the same approach has become
applicable to the level of the proteome, permitting analysis of the turnover of many proteins
instead of single proteins or an aggregated protein pool. The optimal experimental design
for turnover studies is dependent on the nature of the biological system under study, which
dictates the choice of precursor label, protein pool sampling strategy, and treatment of data …