Increasing efforts are directed towards the development of effective vaccines through
induction of virus-specific T cell responses. Although emerging data indicate a significant
role of these cells in determining viral set point in infections such as HIV, there is as yet no
consensus as to the best methods for assaying the breadth of these responses. In this study,
we used sensitive interferon γ-based intracellular cytokine staining (ICS) and Elispot assays
to determine the optimal overlapping peptide set to screen for these responses. Twenty …