[HTML][HTML] Phosphatidylinositol phosphate kinase type 1γ and β1-integrin cytoplasmic domain bind to the same region in the talin FERM domain
IL Barsukov, A Prescot, N Bate, B Patel… - Journal of Biological …, 2003 - ASBMB
Talin is an essential component of focal adhesions that couples β-integrin cytodomains to F-
actin and provides a scaffold for signaling proteins. Recently, the integrin β 3 cytodomain
and phosphatidylinositol phosphate (PIP) kinase type 1γ (a phosphatidylinositol 4, 5-
bisphosphate-synthesizing enzyme) were shown to bind to the talin FERM domain
(subdomain F3). We have characterized the PIP kinase-binding site by NMR using a 15 N-
labeled talin F2F3 polypeptide. A PIP kinase peptide containing the minimal talin-binding …
actin and provides a scaffold for signaling proteins. Recently, the integrin β 3 cytodomain
and phosphatidylinositol phosphate (PIP) kinase type 1γ (a phosphatidylinositol 4, 5-
bisphosphate-synthesizing enzyme) were shown to bind to the talin FERM domain
(subdomain F3). We have characterized the PIP kinase-binding site by NMR using a 15 N-
labeled talin F2F3 polypeptide. A PIP kinase peptide containing the minimal talin-binding …