Enrichment and terminal differentiation of striated muscle progenitors in vitro
UM Becher, M Breitbach, P Sasse, S Garbe… - Experimental cell …, 2009 - Elsevier
UM Becher, M Breitbach, P Sasse, S Garbe, PFM van der Ven, DO Fürst, BK Fleischmann
Experimental cell research, 2009•ElsevierEnrichment and terminal differentiation of mammalian striated muscle cells is severely
hampered by fibroblast overgrowth, de-differentiation and/or lack of functional differentiation.
Herein we report a new, reproducible and simple method to enrich and terminally
differentiate muscle stem cells and progenitors from mice and humans. We show that a
single gamma irradiation of muscle cells induces their massive differentiation into
structurally and functionally intact myotubes and cardiomyocytes and that these cells can be …
hampered by fibroblast overgrowth, de-differentiation and/or lack of functional differentiation.
Herein we report a new, reproducible and simple method to enrich and terminally
differentiate muscle stem cells and progenitors from mice and humans. We show that a
single gamma irradiation of muscle cells induces their massive differentiation into
structurally and functionally intact myotubes and cardiomyocytes and that these cells can be …
Enrichment and terminal differentiation of mammalian striated muscle cells is severely hampered by fibroblast overgrowth, de-differentiation and/or lack of functional differentiation. Herein we report a new, reproducible and simple method to enrich and terminally differentiate muscle stem cells and progenitors from mice and humans. We show that a single gamma irradiation of muscle cells induces their massive differentiation into structurally and functionally intact myotubes and cardiomyocytes and that these cells can be kept in culture for many weeks. Similar results are also obtained when treating skeletal muscle-derived stem cells and progenitors with Mitomycin C.
Elsevier