[HTML][HTML] Designed human serum hyaluronidase 1 variant, HYAL1ΔL, exhibits activity up to pH 5.9
S Reitinger, J Müllegger, B Greiderer… - Journal of biological …, 2009 - ASBMB
Hyaluronidases from diverse species and sources have different pH optima. Distinct
mechanisms with regard to dynamic structural changes, which control hyaluronidase activity
at varying pH, are unknown. Human serum hyaluronidase 1 (HYAL1) is active solely below
pH 5.1. Here we report the design of a HYAL1 variant that degrades hyaluronan up to pH
5.9. Besides highly conserved residues in close proximity of the active site of most
hyaluronidases, we identified a bulky loop formation located at the end of the substrate …
mechanisms with regard to dynamic structural changes, which control hyaluronidase activity
at varying pH, are unknown. Human serum hyaluronidase 1 (HYAL1) is active solely below
pH 5.1. Here we report the design of a HYAL1 variant that degrades hyaluronan up to pH
5.9. Besides highly conserved residues in close proximity of the active site of most
hyaluronidases, we identified a bulky loop formation located at the end of the substrate …