Characterization of human high-density lipoproteins by gradient gel electrophoresis

PJ Blanche, EL Gong, TM Forte, AV Nichols - Biochimica et Biophysica Acta …, 1981 - Elsevier
Gradient gel electrophoresis in conjunction with automated densitometry was applied to the
identification and estimation of subpopulations of high-density lipoproteins (HDL) in the
ultracentrifugal d⩽ 1.200 fraction from human plasma. The frequency distribution of relative
migration distances (RF values) of subpopulation peaks in HDL patterns of a group (n= 194)
of human subjects showed five apparent maxima: two in the RF range associated with the
HDL 2 subclass, and three in the RF range of the HDL 3 subclass. HDL within RF intervals …