Preventive effects of edaravone, a free radical scavenger, on lipopolysaccharide‐induced lung injury in mice
S Tajima, M Soda, M Bando, M Enomoto… - …, 2008 - Wiley Online Library
S Tajima, M Soda, M Bando, M Enomoto, H Yamasawa, S Ohno, T Takada, E Suzuki…
Respirology, 2008•Wiley Online LibraryBackground and objective: Reactive oxygen species (ROS) play an important role in the
pathogenesis of acute lung injury (ALI) and pulmonary fibrosis. It was hypothesized that
edaravone, a free radical scavenger, would be able to attenuate LPS‐induced lung injury in
mice by decreasing oxidative stress. Methods: For the in vivo experiments, lung injury was
induced in female BALB/c mice by the intranasal instillation of LPS. Edaravone was given by
intraperitoneal administration 1 h before the LPS challenge. For the in vitro experiments, MH …
pathogenesis of acute lung injury (ALI) and pulmonary fibrosis. It was hypothesized that
edaravone, a free radical scavenger, would be able to attenuate LPS‐induced lung injury in
mice by decreasing oxidative stress. Methods: For the in vivo experiments, lung injury was
induced in female BALB/c mice by the intranasal instillation of LPS. Edaravone was given by
intraperitoneal administration 1 h before the LPS challenge. For the in vitro experiments, MH …
Background and objective: Reactive oxygen species (ROS) play an important role in the pathogenesis of acute lung injury (ALI) and pulmonary fibrosis. It was hypothesized that edaravone, a free radical scavenger, would be able to attenuate LPS‐induced lung injury in mice by decreasing oxidative stress.
Methods: For the in vivo experiments, lung injury was induced in female BALB/c mice by the intranasal instillation of LPS. Edaravone was given by intraperitoneal administration 1 h before the LPS challenge. For the in vitro experiments, MH‐S cells (murine alveolar macrophage cell line) were exposed to edaravone, followed by stimulation with LPS.
Results: In the LPS‐induced ALI mouse model, the administration of edaravone attenuated cellular infiltration into and the concentrations of albumin, IL‐6, tumour necrosis factor‐α, keratinocyte‐derived chemokine and macrophage inflammatory protein‐2 in BAL fluid. In addition, the in vitro studies showed that the elevated IL‐6 secretion from MH‐S cells in response to LPS was significantly attenuated by co‐incubation with edaravone.
Conclusions: In an experimental murine model, a free radical scavenger may prevent ALI via repression of pro‐inflammatory cytokine production by lung macrophages.
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