[HTML][HTML] A transforming growth factor β (TGFβ) control element drives TGFβ-induced stimulation of smooth muscle α-actin gene expression in concert with two CArG …
MB Hautmann, CS Madsen, GK Owens - Journal of Biological Chemistry, 1997 - ASBMB
The goal of the present study was to determine the molecular mechanism whereby
transforming growth factor β (TGFβ) increases smooth muscle (SM) α-actin expression.
Confluent, growth-arrested rat aortic smooth muscle cells (SMC) were transiently transfected
with various SM α-actin promoter/chloramphenicol acetyltransferase deletion mutants and
stimulated with TGFβ (2.5 ng/ml). Results demonstrated that the first 125 base pairs of the
SM α-actin promoter were sufficient to confer TGFβ responsiveness. Three cis elements …
transforming growth factor β (TGFβ) increases smooth muscle (SM) α-actin expression.
Confluent, growth-arrested rat aortic smooth muscle cells (SMC) were transiently transfected
with various SM α-actin promoter/chloramphenicol acetyltransferase deletion mutants and
stimulated with TGFβ (2.5 ng/ml). Results demonstrated that the first 125 base pairs of the
SM α-actin promoter were sufficient to confer TGFβ responsiveness. Three cis elements …